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VERSION:2.0
PRODID:icalendar-ruby
CALSCALE:GREGORIAN
X-WR-CALNAME:BME 7900: Elliot Botvinick\, UC-Irivine\, Department of Biomed
 ical Engineering and Beckman Laser Institute
X-WR-TIMEZONE:Eastern Time (US & Canada)
BEGIN:VEVENT
DTSTAMP:20260515T052145Z
UID:tag:localist.com\,2008:EventInstance_1062063
DTSTART:20150416T201500Z
DTEND:20150416T211500Z
DESCRIPTION:Feeling pericellular mechanical heterogeneities\n\n \n\nWhile t
 here is strong evidence for roles of bulk stromal stiffness in cell regula
 tion\, roles for the pericellular mechanical microenvironment are less cle
 ar\, in large part due to the difficulty of measurement. My group implemen
 ts automated Active Microrheology (aAMR)\, an optical tweezers technology\
 , to probe extracellular stiffness and map it in the volume surrounding ce
 lls. Our aAMR applies sinusoidal optical forces onto microbeads embedded w
 ithin natural extracellular matrices (ECMs)\, including those comprised of
  fibrin and type 1 collagen. As in the case of passive microrheology\, aAM
 R reports the complex material response function of the ECM just surroundi
 ng each microbead. Different from passive methods\, aAMR is valid for syst
 ems not in thermal equilibrium\, as is typical for regions of the ECM near
  to contractile cells. Our aAMR microscope can probe many beads surroundin
 g each cell to map the mechanical landscape\, allowing us to seek correlat
 ions between local stiffness distributions and cell properties such as con
 tractility\, signaling\, and differentiation. I will present specific exam
 ples for which the distribution of pericellular stiffness correlates with 
 cell phenotype/state including: MT1-MMP deficient mesenchymal stem cells\,
  human aortic smooth muscle cells with compromised contractility and fibro
 sarcoma cells cultured in type 1 collagen extracted by several methods.
GEO:42.446873;-76.477196
LOCATION:Weill Hall\, 226
SUMMARY:BME 7900: Elliot Botvinick\, UC-Irivine\, Department of Biomedical 
 Engineering and Beckman Laser Institute
URL;VALUE=URI:https://events.cornell.edu/event/bme_7900_elliot_botvinick_uc
 -irivine_department_of_biomedical_engineering_and_beckman_laser_institute
CATEGORIES:Lecture
CATEGORIES:Seminar
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