Abstract: Cell migration underlies many physiological processes ranging from immune response to wound healing and to cancer invasion. Diverse environmental factors such as chemical gradients and electric fields can guide the migration and trafficking of various cell types in tissues. Rapid development of microfluidics provides powerful quantitative test bed for cell migration analysis in controlled cellular microenvironments. Toward this direction, we focus on using microfluidic devices to study chemotactic and electrotactic migration of different cell types. In this talk, I will first provide relevant background for this research field followed by an overview of my research program. Then I will focus on introducing our electrotaxis studies including 1) lymphocyte electrotaxis analysis in vitro and in vivo; 2) experimental and modeling studies of immune cell migration in competing chemical gradient and electric field; 3) electrotaxis analysis of cancer cells and stem cells.