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Wednesday, June 13, 2018 at 12:20pm to 1:10pm
Switchgrass (Panicum virgatum L.) suffered from germination reduction from Bipolaris seed rot (BSR) and in yield reduction from Bipolaris leaf spot (BLS) caused by a necrotrophic ascomycete fungus named Bipolaris oryzae (Breda de Haan) Shoemaker. To manage the diseases breeding, switchgrass for resistance and silicon amendment were two potential approaches. Screening methods in a greenhouse were established for BSR in seeds and BLS in 4-week-old seedlings. The progress of resistance to BSR was over 50% cycle-1 in both cultivars whereas the progress of resistance to BLS was not significant. To dissect the resistance to BLS, a multi-trait Genome-wide association was conducted in Northern association panel. The BLS evaluation was conducted in 479 mature switchgrass via field evaluation, leaf detachment, and disk leaf assay. Multi-trait GWAS from different phenotype combinations revealed potential resistance genes encoding Myb, cytochrome P450, isocitrate lyase, E3 ubiquitin-protein, etc. Nevertheless, silicon amendment showed no improvement of resistance to both BSR and BLS