Wednesday, November 15, 2017 at 4:00pm to 5:00pm
Clark Hall, 700
Lauren Jackson, Vanderbilt University
Host: Chris Fromme
Mechanisms of coat assembly & regulation in membrane trafficking pathways
Membrane trafficking pathways are vital to eukaryotic cell health and viability. The Jackson lab investigates molecular structures and functions of coat protein complexes that initiate cellular trafficking pathways. We focus on the roles of the adaptor protein 4 (AP4), retromer, and coat protein complex I (COPI) coats in both fundamental cell biology and human brain disease. Coat protein complexes function as “hubs” by recognizing cargo and coordinating large protein networks that drive regulated formation of vesicles or tubules at donor membranes. We use a variety of biochemical, biophysical, and structural methods to address at the molecular level how coats interact with protein and lipid partners to initiate and regulate coat assembly and sort cargoes to different destinations. In my talk, I will highlight recently published and ongoing work on AP4 coats (Frazier et al, Traffic 2016; Archuleta et al, Traffic 2017). I will also present two unpublished stories: electron microscopy data supporting how mammalian retromer assembles, and biochemical data on the ArfGAP, Glo3, interacts with the COPI B-subcomplex.