Biophysics Colloquium with Ilya Finkelstein

Wednesday, May 3, 2017 at 4:00pm to 5:00pm

Clark Hall, 700
Central Campus

Ilya Finkelstein, University of Texas

Host: Roger Loring

Single-molecule views of bacterial adaptive immunity

Clustered regularly interspaced short palindromic repeats (CRISPRs) are a recently discovered RNA-based adaptive immune system that protects bacteria and archaea from foreign DNA and play a central role in controlling horizontal gene transfer. More recently, CRISPR-associated (cas) proteins have been harnessed for programmable gene regulation and precision genome editing. All CRISPR systems require complementary base pairing between an RNA-guided nucleoprotein complex and the target DNA. Off-target activities have also been reported, but we currently lack a comprehensive and quantitative description of sequence-dependent DNA binding and nuclease activation. I will discuss our recent progress in using a combination of single-molecule biophysics and next-generation sequencing to uncover the mechanisms of target recognition and DNA degradation by a variety of CRISPR systems. This work offers new insights in understanding the mechanisms of CRISPR-associated adaptive immunity and for using these enzymes for precision genome engineering in both scientific and future therapeutic settings.

poster

Event Type

Seminar

Departments

LASSP

Tags

biophysics, biophysics_colloquium, lassp

Contact E-Mail

jdf55@cornell.edu

Contact Name

Jonathan Fuller

Contact Phone

255-8101

Speaker

Ilya Finkelstein

Speaker Affiliation

University of Texas

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