Wednesday, October 4, 2017 at 4:00pm to 5:00pm
Clark Hall, 700
Hong Li, Florida State University
Host: Ailong Ke
Learning the Rules of Substrate Specificity in RNA-guided Enzymes
Small non-coding RNA can act as effective guides for protein enzymes in mounting chemical changes on large nucleic acid substrates, such as ribosomal RNA and viral genomes. The programmability of the RNA-guided enzymes makes them attractive targets for developing nucleic acid detection and genome science tools. Unlike protein-only enzymes, the RNA-guided enzymes bind substrates through Watson-Crick base pairing and thus have unique set of rules to impart specificity. Three examples RNA-guided enzymes, an RNA 2'-OH methyltranferase, a pseudouridylase, and a DNA endonuclease, will be discussed to explore substrate specificity of these unique enzymes. We learned that RNA structure, solvent accessible area of the target, and DNA topology can influence substrate specificity.